| # | Title | Artist | Rating | Length |
|---|
| 1 | Lonesome Valley | The Monroe Brothers | | 2:13 |
| 2 | Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | | 3:08 |
| 3 | My Dixie Darling | The Carter Family | | 2:58 |
| 4 | I'll Fly Away | Brown’s Ferry Four | | 2:58 |
| 5 | Tom Cat Blues | Cliff Carlisle | 5 | 2:54 |
| 6 | Roll All Burdens Away | The Fairfield Four | | 2:39 |
| 7 | Coo Coo Bird | Clarence Ashley | 3.75 | 2:57 |
| 8 | Rounder's Blues | The Delmore Brothers | | 2:57 |
| 9 | California Blues (Blue Yodel No. 4) | Jimmie Rodgers | 4 | 3:05 |
| 10 | Stop and Listen Blues | The Mississippi Sheiks | | 3:34 |
| 11 | I Got Your Ice Cold Nugrape | Nugrape Twins | | 2:56 |
| 12 | The Rooster's Crowing Blues | Cannon’s Jug Stompers | 5 | 3:02 |
| 13 | I Looked Down the Line | The Dixie Hummingbirds | | 2:48 |
| 14 | The Dying Soldier | Buell Kazee | | 3:07 |
| 15 | Wildwood Flower | The Carter Family | 4 | 3:12 |
| 16 | Standing in the Safety Zone | The Fairfield Four | | 2:47 |
| 17 | Danville Girl | Dock Boggs | | 3:10 |
| 18 | Keep Your Lamp Trimmed and Burning | Blind Willie Johnson | | 3:05 |
| 19 | Blue Grass Special | Bill Monroe & His Blue Grass Boys | | 2:31 |
| 20 | Ride Old Buck to Water | Gid Tanner & The Skillet Lickers | | 3:07 |
| 21 | Mississippi Bo Weavil | Charley Patton | | 3:11 |
| 22 | Take a Drink on Me | Charlie Poole | | 3:15 |
| 23 | Lonesome Valley | David Miller | | 2:48 |
| 24 | I'm a Man of Constant Sorrow | The Stanley Brothers | | 3:02 |